DTC P0300-00: Random/Multiple Cylinder Misfire Detected; DTC P0300-27: Random/Multiple Cylinder Misfire Detected (Emission) Signal Rate of Change Above Threshold; DTC P0300-85: Random / Multiple Cylinder Misfire Detected (Over Temperature) Signal Above Allowable Range; DTC P0301-00: Cylinder 1 Misfire Detected; DTC P0302-00: Cylinder 2 Misfire Detected; DTC P0303-00: Cylinder 3 Misfire Detected; DTC P0304-00: Cylinder 4 Misfire Detected [11/2023 - ]: Procedure

2025 Toyota Highlander XLE, FWDSECTION Procedure
  1. CHECK ANY OTHER DTCS OUTPUT (IN ADDITION TO MISFIRE DTCS) 
    1. Read the DTCs.

      Powertrain > Engine > Trouble Codes 

      Result

      Result Proceed to
      DTC P0300-00, P0300-27, P0300-85, P0301-00, P0302-00, P0303-00 and/or P0304-00 are output A
      DTC P0300-00, P0300-27, P0300-85, P0301-00, P0302-00, P0303-00 and/or P0304-00 and other DTCs are output B

      HINT: 

      If any DTCs other than P0300-00, P0300-27, P0300-85, P0301-00, P0302-00, P0303-00 and/or P0304-00 are output, troubleshoot those DTCs first.

    Result: 

    GO TO DTC CHART. Refer to  DIAGNOSTIC TROUBLE CODE CHART [11/2023 - ] 

    Result: 

    See step  2

  2. CHECK PCV VALVE AND HOSE CONNECTIONS 
    GTY1108210Courtesy of © TOYOTA, LICENSE AGREEMENT TMS1002
    1. Check the PCV hose connections.
    2. Check the PCV valve.

      Refer to INSPECTION [10/2022 - ]

      OK

      PCV hose and PCV valve are connected correctly and are not damaged.

      Result

      Proceed to
      OK
      NG

    Result: 

    NG 

    REPAIR OR REPLACE PCV VALVE OR HOSE 

    Result: 

    OK 

    See step  3

  3. READ VALUE USING GTS (MISFIRE RPM AND MISFIRE LOAD) 
    1. Read and note the Misfire RPM and Misfire Load values.

      Powertrain > Engine > Data List 

      Tester Display
      Misfire RPM
      Misfire Load

      HINT: 

      The Misfire RPM and Misfire Load values indicate the vehicle conditions under which the misfire occurred.

      Result

      Proceed to
      NEXT

    Result: 

    NEXT 

    See step  4

  4. READ VALUE USING GTS (CATALYST OT MISFIRE FUEL CUT) 
    1. Read the value displayed on the GTS.

      Powertrain > Engine > Data List 

      Tester Display
      Catalyst OT Misfire Fuel Cut

      Result

      Data List Result Proceed to
      Catalyst OT Misfire Fuel Cut Avail A
      Not Avi B

    Result: 

    See step  7

    Result: 

    See step  5

  5. READ FREEZE FRAME DATA (CATALYST OT MISFIRE FUEL CUT HISTORY) 
    1. Using the GTS, confirm the vehicle conditions recorded in the Freeze Frame Data which were present when the DTC was stored.

      Refer to FREEZE FRAME DATA [11/2023 - ]

      Result

      Freeze Frame Data Item Result Proceed to
      Catalyst OT Misfire Fuel Cut History ON A
      OFF B

    Result: 

    See step  7

    Result: 

    See step  6

  6. CHECK MISFIRE COUNT OF PORT INJECTION 
    1. Start the engine and warm it up until the engine coolant temperature reaches 75°C (167°F) or higher.

      Powertrain > Engine > Data List 

      Tester Display
      Coolant Temperature
    2. Read each value for Misfire Count Cylinder #1 to Misfire Count Cylinder #4 and Injection Mode displayed on the GTS while performing the Active Test.

      Powertrain > Engine > Active Test 

      Active Test Display
      Prohibit the Catalyst OT Misfire Prevent Fuel Cut
      Data List Display
      Injection Mode
      Misfire Count Cylinder #1
      Misfire Count Cylinder #2
      Misfire Count Cylinder #3
      Misfire Count Cylinder #4

      Result

      Injection Mode Misfire Count Proceed to
      Port 1 or 2 cylinders have misfire counts A
      3 cylinders or more have misfire counts
      There are no misfire counts B

    Result: 

    See step  8

    Result: 

    See step  11

  7. CHECK MISFIRE COUNT OF PORT INJECTION 
    1. Start the engine and warm it up until the engine coolant temperature reaches 80°C (176°F) or higher.

      Powertrain > Engine > Data List 

      Tester Display
      Coolant Temperature
    2. According to the display on the GTS, read the Data List with the Active Test "Control the Injection Mode" set to Port. If no misfire counts occur in any cylinders, perform procedure [A] and [B] and then check the misfire counts again.

      Powertrain > Engine > Active Test 

      Active Test Display
      Control the Injection Mode
      Data List Display
      Injection Mode
      Misfire Count Cylinder #1
      Misfire Count Cylinder #2
      Misfire Count Cylinder #3
      Misfire Count Cylinder #4
      1. Drive the vehicle with the Misfire RPM and Misfire Load noted in the "Read Value Using GTS (Misfire RPM and Misfire Load)" procedures above [A].
      2. Read the Injection Mode and Misfire Count Cylinder #1 to Misfire Count Cylinder #4 or DTCs displayed on the GTS [B].

      Result

      Injection Mode Misfire Count Proceed to
      Port 1 or 2 cylinders have misfire counts A
      3 cylinders or more have misfire counts
      There are no misfire counts B

    Result: 

    See step  11

    Result: 

    See step  8

  8. CHECK MISFIRE COUNT OF DIRECT INJECTION 
    1. Start the engine and warm it up until the engine coolant temperature reaches 80°C (176°F) or higher.

      Powertrain > Engine > Data List 

      Tester Display
      Coolant Temperature
    2. According to the display on the GTS, read the Data List with the Active Test "Control the Injection Mode" set to Direct. If no misfire counts occur in any cylinders, perform procedure [A] and [B] and then check the misfire counts again.

      Powertrain > Engine > Active Test 

      Active Test Display
      Control the Injection Mode
      Data List Display
      Injection Mode
      Misfire Count Cylinder #1
      Misfire Count Cylinder #2
      Misfire Count Cylinder #3
      Misfire Count Cylinder #4
      1. Drive the vehicle with the Misfire RPM and Misfire Load noted in the "Read Value Using GTS (Misfire RPM and Misfire Load)" procedures above [A].
      2. Read the Injection Mode and Misfire Count Cylinder #1 to Misfire Count Cylinder #4 or DTCs displayed on the GTS [B].

      Result

      Misfire Count Proceed to
      Port (Result of step 6 or 7) Direct
      1 or 2 cylinders have misfire counts 1 or 2 cylinders have misfire counts A
      3 cylinders or more have misfire counts 3 cylinders or more have misfire counts B
      1 or 2 cylinders have misfire counts There are no misfire counts C
      3 cylinders or more have misfire counts There are no misfire counts D

      HINT: 

      Perform "Inspection After Repair" after replacing the port fuel injector assembly.

      Refer to INITIALIZATION [10/2022 - ]

    Result: 

    See step  14

    Result: 

    See step  17

    Result: 

    REPLACE PORT FUEL INJECTOR ASSEMBLY (MISFIRING CYLINDER). Refer to  REMOVAL [11/2023 - ] 

    Result: 

    See step  9

  9. CHECK FUEL PRESSURE (FOR LOW PRESSURE SIDE) 
    1. Check the fuel pressure (for low pressure side).

      Refer to PROCEDURE - Step 2

      HINT: 

      Perform "Inspection After Repair" after replacing the port fuel injector assembly.

      Refer to INITIALIZATION [10/2022 - ]

      Result

      Proceed to
      OK
      NG

    Result: 

    OK 

    REPLACE PORT FUEL INJECTOR ASSEMBLY (MISFIRING CYLINDER). Refer to  REMOVAL [11/2023 - ] 

    Result: 

    NG 

    See step  10

  10. CHECK FUEL LINE 
    1. Check the fuel lines for leaks or blockage.

      HINT: 

      Perform "Inspection After Repair" after replacing the fuel pump (for low pressure side).

      Refer to INITIALIZATION [10/2022 - ]

      Result

      Proceed to
      OK
      NG

    Result: 

    OK 

    GO TO FUEL PUMP CONTROL CIRCUIT. Refer to  Fuel Pump Control Circuit [10/2022 - ] 

    Result: 

    NG 

    REPAIR OR REPLACE FUEL SYSTEM 

  11. CHECK MISFIRE COUNT OF DIRECT INJECTION 
    1. Start the engine and warm it up until the engine coolant temperature reaches 80°C (176°F) or higher.

      Powertrain > Engine > Data List 

      Tester Display
      Coolant Temperature
    2. According to the display on the GTS, read the Data List with the Active Test "Control the Injection Mode" set to Direct. If no misfire counts occur in any cylinders, perform procedure [A] and [B] and then check the misfire counts again.

      Powertrain > Engine > Active Test 

      Active Test Display
      Control the Injection Mode
      Data List Display
      Injection Mode
      Misfire Count Cylinder #1
      Misfire Count Cylinder #2
      Misfire Count Cylinder #3
      Misfire Count Cylinder #4
      1. Drive the vehicle with the Misfire RPM and Misfire Load noted in the "Read Value Using GTS (Misfire RPM and Misfire Load)" procedures above [A].
      2. Read the Injection Mode and Misfire Count Cylinder #1 to Misfire Count Cylinder #4 or DTCs displayed on the GTS [B].

      Result

      Misfire Count Proceed to
      Port (Result of step 6 or 7) Direct
      There are no misfire counts There are misfire counts A
      There are no misfire counts There are no misfire counts B

      HINT: 

      Perform "Inspection After Repair" after replacing the direct fuel injector assembly.

      Refer to INITIALIZATION [10/2022 - ]

    Result: 

    REPLACE DIRECT FUEL INJECTOR ASSEMBLY. Refer to  REMOVAL [11/2023 - ] 

    Result: 

    See step  12

  12. CLEAR DTC 
    1. Clear the DTCs.

      Powertrain > Engine > Clear DTCs 

    2. Turn the ignition switch off and wait for at least 30 seconds.

      Result

      Proceed to
      NEXT

    Result: 

    NEXT 

    See step  13

  13. CHECK MISFIRE COUNT 
    1. Start the engine and warm it up until the engine coolant temperature reaches 75°C (167°F) or higher.

      Powertrain > Engine > Data List 

      Tester Display
      Coolant Temperature
    2. Drive the vehicle with Misfire RPM and Misfire Load.

      Powertrain > Engine > Data List 

      Tester Display
      Injection Mode
      Misfire Count Cylinder #1
      Misfire Count Cylinder #2
      Misfire Count Cylinder #3
      Misfire Count Cylinder #4
      Misfire RPM
      Misfire Load
    3. Monitor all of the misfire count values and Injection Mode displayed on the GTS.

      Result

      Misfire Count Injection Mode Proceed to
      There are no misfire counts - A
      1 or 2 cylinders have misfire counts Port or Either B
      3 cylinders or more have misfire counts Port or Either C
      There are misfire counts Direct D

      HINT: 

      Perform "Inspection After Repair" after replacing the direct fuel injector assembly.

      Refer to INITIALIZATION [10/2022 - ]

    Result: 

    CHECK FOR INTERMITTENT PROBLEMS. Refer to  CHECK FOR INTERMITTENT PROBLEMS [10/2022 - ] 

    Result: 

    See step  17

    Result: 

    REPLACE DIRECT FUEL INJECTOR ASSEMBLY. Refer to  REMOVAL [11/2023 - ] 

    Result: 

    See step  14

  14. INSPECT SPARK PLUG 
    1. Inspect the spark plug of the misfiring cylinder.

      Refer to PROCEDURE - Step 3

      HINT: 

      Perform "Inspection After Repair" after replacing the spark plug.

      Refer to INITIALIZATION [10/2022 - ]

      Result

      Proceed to
      OK
      NG

    Result: 

    NG 

    REPLACE SPARK PLUG. Refer to  REMOVAL [10/2022 - ] 

    Result: 

    OK 

    See step  15

  15. CHECK FOR SPARK (SPARK TEST) 
    1. Perform a spark test.

      Refer to PROCEDURE - Step 1

      HINT: 

      • If the result of the spark test is normal, proceed to the next step.
      • Perform "Inspection After Repair" after replacing the spark plug or ignition coil assembly.

        Refer to INITIALIZATION [10/2022 - ]

      Result

      Proceed to
      NEXT

    Result: 

    NEXT 

    See step  16

  16. CHECK CYLINDER COMPRESSION PRESSURE 
    1. Measure the cylinder compression pressure of the misfiring cylinder.

      Refer to PROCEDURE - Step 11

      HINT: 

      Perform "Inspection After Repair" after repairing or replacing the engine assembly.

      Refer to INITIALIZATION [10/2022 - ]

      Result

      Proceed to
      OK
      NG

    Result: 

    OK 

    REPLACE ECM. Refer to  REMOVAL [11/2023 - ] 

    Result: 

    NG 

    CHECK ENGINE TO DETERMINE CAUSE OF LOW COMPRESSION 

  17. CHECK INTAKE SYSTEM 
    1. Check the intake system for vacuum leaks.

      Refer to PROCEDURE - Step 1

      OK

      No leaks from intake system.

      HINT: 

      Perform "Inspection After Repair" after repairing or replacing the intake system.

      Refer to INITIALIZATION [10/2022 - ]

      Result

      Proceed to
      OK
      NG

    Result: 

    NG 

    REPAIR OR REPLACE INTAKE SYSTEM 

    Result: 

    OK 

    See step  18

  18. READ VALUE USING GTS (COOLANT TEMPERATURE) 
    1. Read the Data List twice, when the engine is both cold and warmed up.

      Powertrain > Engine > Data List 

      Tester Display
      Coolant Temperature

      Standard

      GTS Display Condition Specified Condition
      Coolant Temperature Cold engine Same as ambient air temperature
      Warm engine Between 75 and 115°C (167 and 239°F)

      HINT: 

      Perform "Inspection After Repair" after replacing the engine coolant temperature sensor.

      Refer to INITIALIZATION [10/2022 - ]

      Result

      Proceed to
      OK
      NG

    Result: 

    NG 

    REPLACE ENGINE COOLANT TEMPERATURE SENSOR. Refer to  REMOVAL [11/2023 - ] 

    Result: 

    OK 

    See step  19

  19. READ VALUE USING GTS (MASS AIR FLOW SENSOR) 
    1. Start the engine.
    2. Allow the engine to idle until Coolant Temperature reaches 75°C (167°F) or higher.

      Powertrain > Engine > Data List 

      Tester Display
      Coolant Temperature
    3. Read Mass Air Flow Sensor while maintaining an engine speed of 3000 rpm.

      Powertrain > Engine > Data List 

      Tester Display
      Engine Speed
      Mass Air Flow Sensor

      Standard

      GTS Display Condition Specified Condition
      Mass Air Flow Sensor Engine warmed up
      Shift position: P
      A/C: Off
      Engine Speed: 3000 rpm
      Between 5 and 15 gm/sec

      Result

      Proceed to
      OK
      NG

    Result: 

    NG 

    See step  24

    Result: 

    OK 

    See step  20

  20. CHECK VALVE TIMING (CHECK FOR LOOSE TIMING CHAIN AND JUMPED TEETH) 
    1. Remove the cylinder head cover sub-assembly.
      GTY1076329Courtesy of © TOYOTA, LICENSE AGREEMENT TMS1002
      *a 10.6° *b 21.91°
      *c Timing Mark *d "0" Timing Mark
      *e Groove - -
    2. Turn the crankshaft pulley and align its groove with the "0" timing mark of the timing chain cover.
    3. Check that the timing marks of the camshaft timing gear assembly and camshaft timing exhaust gear assembly are at the positions shown in the illustration.

      HINT: 

      If the timing marks are not at the positions shown, turn the crankshaft one revolution clockwise.

      OK

      Timing marks on camshaft timing gear assembly and camshaft timing exhaust gear assembly are at the positions shown in the illustration.

      HINT: 

      If the result is not as specified, check for mechanical malfunctions that may have affected the valve timing, such as a jumped tooth or stretching of the timing chain.

      Result

      Proceed to
      OK
      NG

    Result: 

    OK 

    See step  24

    Result: 

    NG 

    See step  21

  21. CHECK ENGINE MECHANICAL SYSTEM 
    1. Check for mechanical malfunctions that affect the valve timing, such as a jumped tooth or stretching of the timing chain.

      HINT: 

      Perform "Inspection After Repair" after repairing or replacing the engine mechanical system.

      Refer to INITIALIZATION [10/2022 - ]

      Result

      Proceed to
      OK
      NG

    Result: 

    NG 

    REPAIR OR REPLACE MALFUNCTIONING PARTS, COMPONENT AND AREA 

    Result: 

    OK 

    See step  22

  22. CLEAR DTC 
    1. Clear the DTCs.

      Powertrain > Engine > Clear DTCs 

    2. Turn the ignition switch off and wait for at least 30 seconds.

      Result

      Proceed to
      NEXT

    Result: 

    NEXT 

    See step  23

  23. CONFIRM WHETHER DTC OUTPUT RECURS (MISFIRE DTCS) 
    1. Drive the vehicle in accordance with the driving pattern described in Confirmation Driving Pattern.
    2. Check the DTC judgment result.

      Powertrain > Engine > Utility 

      Tester Display
      All Readiness
    3. Input the DTC: P0300-00, P0300-27, P0300-85, P0301-00, P0302-00, P0303-00 or P0304-00.

      Result

      Result Proceed to
      NORMAL
      (DTCs are not output)
      A
      ABNORMAL
      (DTC P0300-00, P0300-27, P0300-85, P0301-00, P0302-00, P0303-00 and/or P0304-00 is output)
      B

    Result: 

    CHECK FOR INTERMITTENT PROBLEMS. Refer to  CHECK FOR INTERMITTENT PROBLEMS [10/2022 - ] 

    Result: 

    See step  24

  24. CHECK HARNESS AND CONNECTOR (MASS AIR FLOW METER SUB-ASSEMBLY CONNECTOR CONNECTION) 
    1. Check the connection and terminal contact pressure of connectors and wire harnesses between the mass air flow meter sub-assembly and ECM.

      Refer to ELECTRONIC CIRCUIT INSPECTION PROCEDURE [12/2019 - ]

      HINT: 

      Repair any problems.

      Result

      Proceed to
      NEXT

    Result: 

    NEXT 

    See step  25

  25. CLEAR DTC 
    1. Clear the DTCs.

      Powertrain > Engine > Clear DTCs 

    2. Turn the ignition switch off and wait for at least 30 seconds.

      Result

      Proceed to
      NEXT

    Result: 

    NEXT 

    See step  26

  26. CHECK WHETHER DTC OUTPUT RECURS (MISFIRE DTCS) 
    1. Drive the vehicle in accordance with the driving pattern described in Confirmation Driving Pattern.
    2. Check the DTC judgment result.

      Powertrain > Engine > Utility 

      Tester Display
      All Readiness
    3. Input the DTC: P0300-00, P0300-27, P0300-85, P0301-00, P0302-00, P0303-00 or P0304-00.

      Result

      Result Proceed to
      NORMAL
      (DTCs are not output)
      A
      ABNORMAL
      (DTC P0300-00, P0300-27, P0300-85, P0301-00, P0302-00, P0303-00 and/or P0304-00 is output)
      B

    Result: 

    END 

    Result: 

    See step  27

  27. CHECK HARNESS AND CONNECTOR (MASS AIR FLOW METER SUB-ASSEMBLY - ECM) 
    1. Disconnect the mass air flow meter sub-assembly connector.
    2. Disconnect the ECM connector.
    3. Measure the resistance according to the value(s) in the table below.

      Standard Resistance

      Tester Connection Condition Specified Condition
      C30-3 (VCC) - C88-73 (VCVG) Always Below 1 Ω
      C30-1 (FG) - C88-75 (VG) Always Below 1 Ω
      C30-2 (E2G) - C88-74 (E2G) Always Below 1 Ω
      C30-3 (VCC) or C88-73 (VCVG) - Body ground and other terminals Always 10 kΩ or higher
      C30-1 (FG) or C88-75 (VG) - Body ground and other terminals Always 10 kΩ or higher
      C30-2 (E2G) or C88-74 (E2G) - Body ground and other terminals Always 10 kΩ or higher

      Result

      Proceed to
      OK
      NG

    Result: 

    NG 

    REPAIR OR REPLACE HARNESS OR CONNECTOR 

    Result: 

    OK 

    See step  28

  28. REPLACE MASS AIR FLOW METER SUB-ASSEMBLY 
    1. Replace the mass air flow meter sub-assembly.

      Refer to REMOVAL [10/2022 - ]

      HINT: 

      • If the results of the inspections performed in step 19 (READ VALUE USING GTS (MASS AIR FLOW SENSOR)) indicated no problem, proceed to the next step without replacing the mass air flow meter sub-assembly.
      • Perform "Inspection After Repair" after replacing the mass air flow meter sub-assembly.

        Refer to INITIALIZATION [10/2022 - ]

      Result

      Proceed to
      NEXT

    Result: 

    NEXT 

    See step  29

  29. CLEAR DTC 
    1. Clear the DTCs.

      Powertrain > Engine > Clear DTCs 

    2. Turn the ignition switch off and wait for at least 30 seconds.

      Result

      Proceed to
      NEXT

    Result: 

    NEXT 

    See step  30

  30. CONFIRM WHETHER MALFUNCTION HAS BEEN SUCCESSFULLY REPAIRED 
    1. Drive the vehicle in accordance with the driving pattern described in Confirmation Driving Pattern.
    2. Check the DTC judgment result.

      Powertrain > Engine > Utility 

      Tester Display
      All Readiness
    3. Input the DTC: P0300-00, P0300-27, P0300-85, P0301-00, P0302-00, P0303-00 or P0304-00.

      Result

      Result Proceed to
      NORMAL
      (DTCs are not output)
      A
      ABNORMAL
      (DTC P0300-00, P0300-27, P0300-85, P0301-00, P0302-00, P0303-00 and/or P0304-00 is output)
      B

    Result: 

    END 

    Result: 

    REPLACE ECM. Refer to  REMOVAL [11/2023 - ] 

RENDER: 1.0x

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When to See a Mechanic

Stop DIY work and contact a certified mechanic immediately if any of the following apply:

  • You smell fuel, burning insulation, or see smoke.
  • Brakes feel soft, pull hard to one side, or make grinding noises.
  • The engine overheats, stalls repeatedly, or misfires under load.
  • You are missing required tools, torque specs, or safe lifting equipment.
  • You are not confident in the next step or safety outcome.